Open Positions


Research Associate

Cell Biology

Spotlight is hiring RA/SRA in the platform development group. An ideal
candidate should be extremely proficient in cell culture, maintaining and transfecting various mouse and human cell lines. Candidate should also be comfortable handling mice post necropsy. This position supports the work of gene editing scientists as well as multidisciplinary Spotlight groups
The candidate should have a minimum of 1-2 years of industry experience or at least 4 years of academic experience in cell biology and assay development. Experience using CRISPR/Cas system and developing biologics, especially antibody-based therapeutics is an added bonus.

 

RESPONSIBILTIES

  • Culture, maintain and transfect immortalized and primary human and mouse cells.
  • Perform various in vitro and in vivo assays.
  • Design and analyze CRISPR/Cas genome editing experiments.
  • Design and execute small animal (mouse) in vivo experiments.
  • Systematically carry out mouse tissue dissections post necropsy.
  • Prepare tissue samples for staining (fixation, embedding, sectioning).
  • Carry out staining and imaging of cells and tissues using IF/IHC methods.
  • Independently analyze, interpret data and troubleshoot experiments and protocols as
    required.

EXPERIENCE

  • Hands on experience in primary cell culture, cell-based assays, biochemical assays, flow cytometry and cell sorting, design and execution of in vivo experiments, and general cell and molecular biology techniques
  • Fluorescence microscopy, live-cell microscopy and/or FACSs
  • Ability to independently analyze and interpret data (e.g., GraphPad Prism, excel etc)
  • Ability to work with animals and handle mouse and human tissues
  • Manage multiple tasks efficiently and communicate clearly

QUALIFICATIONS

Minimum B.S. preferably in biological sciences with 4+ years relevant industrial
or academic experience in the areas of Cellular Biology, Cellular Immunology or related field experience; or MA or MS in the Life Sciences with more than 2 years relevant industry
laboratory experience

How to apply

To apply, send a copy of your CV to careers@spotlighttx.com
 
This hiring window will be open until August 31st, 2018. 


Research Associate

Molecular Biology

We are hiring a Research Associate/Senior Research Associate (Bachelor’s or Master’s degree in a related field) to take our molecular biology workflows from whiteboard conceptualization to laboratory scale implementation. This position will support Scientists in our protein engineering group and will interact with other scientific groups within Spotlight.
 

The position will be responsible for the design, optimization, and cloning of bacterial, eukaryotic (S. cerevisiae) and mammalian (CHO, HEK-293/F) expression plasmids. The ideal candidate will be well-versed in cloning methodologies ranging from traditional restriction cloning to Gibson assembly to USER/GoldenGate approaches.

Experience with the molecular biology behind protein variant library generation, protein linker optimization, in-vitro transcription-translation, and various display technologies such as yeast and phage display techniques and Next-gen sequencing/Illumina workflows are added pluses.  

RESPONSIBILITIES

  • Design, optimization, and cloning of bacterial and eukaryotic expression plasmids.
  • Growth and induction of expression cultures.
  • Assistance with protein variant screening to discover new biotherapeutics.
     

EXPERIENCE

  • RA candidates should have at least two years of relevant experience.
  • SRA candidates should have 5+ years of relevant experience.
  • Broad knowledge of cloning techniques (restriction cloning, Gibson assembly, Type-IIS, USER/GoldenGate, Ligation independent) required.
  • Experience with protein variant library construction and screening desired.
  • Experience with display technologies (yeast/phage) desired.
  • Illumina sequencing experience desired. 
  • Flow cytometry experience desired.
     

QUALIFICATIONS

Bachelor’s or advanced degree

How to Apply

To apply, send a copy of your CV to careers@spotlighttx.com

This hiring window will be open until August 31st, 2018.